Determining the phoM map location in Escherichia coli K-12 by using a nearby transposon Tn10 insertion

Resistance to various tetracyclines mediated by transposon Tn10 in Escherichia coli K-12.

Levels of resistance to tetracycline, chlortetracycline, demethylchlortetracycline, doxycycline, oxytetracycline, methacycline, pyrrolidinotetracycline, minocycline, and beta-chelocardin of Escherichia coli K-12 carrying transposon Tn10 or defined DNA segments of Tn10 were determined. In all cases, tetA was the only gene required for resistance. Doxycycline was the most effective inducer of tet...

Deletions generated by the transposon Tn10 in the srl recA region of the Escherichia coli K-12 chromosome.

A negative regulatory gene for the srl operon (srlR) was recognized by the characteristics of an insertion mutation generated by the transposon Tn10 determining tetracycline resistance. This finding is discussed in light of previous hypotheses on the regulation of the srl genes, which mediate metabolism of glucitol (i.e., sorbitol). Mapping showed that the order of genes in this region is: srlR...

Escherichia coli K-12 mutation that inactivates biodegradative threonine dehydratase by transposon Tn5 insertion.

From a collection of kanamycin-resistant mutants of Escherichia coli K-12 isolated by transposon Tn5 mutagenesis, we have identified a mutant that lacks functional biodegradative threonine dehydratase (EC 4.2.1.16) by direct enzyme assay and by the loss of cross-reacting material with affinity-purified antibodies against the purified enzyme. Aerobic and anaerobic growth of this strain on variou...

Physical map location of the asc (formerly sac) operon of Escherichia coli K-12.

The Genetic Map of Escherichia Coli K-12.

OF the several known methods for locating the positions of genetic markers on the single linkage group of Escherichia coli, the one most convenient and satisfactory for mapping long chromosomal segments is the interrupted conjugation technique described by WOLLMAN, JACOB and HAYES (1956). This method uses strong mechanical agitation to sever the conjugation tube that unites mating cell pairs. A...